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This Article Full Text (Papers in Press[PDF]) All Versions of this Article: vir.0.014118-0v1 90/11/2746    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dortmans, J. C.F.M. Articles by Peeters, B. P Search for Related Content PubMed PubMed Citation Articles by Dortmans, J. C.F.M. Articles by Peeters, B. P Agricola Articles by Dortmans, J. C.F.M. Articles by Peeters, B. P

Virulence of pigeon paramyxovirus type 1 (PPMV-1) not always correlates with the cleavability of its fusion protein

¹ Central Veterinary Institute of Wageningen UR;
² Utrecht University

³ E-mail: jos.dortmans{at}wur.nl

Some pigeon paramyxovirus type 1 (PPMV-1) strains exhibit low virulence in chickens despite their F protein's multi-basic cleavage site. To elucidate the molecular basis of the low pathogenicity of these strains we constructed an infectious full-length cDNA clone of PPMV-1 strain AV324. This strain is non-virulent for chickens though its F protein contains the typical virulence motif 112RRKKRF117. Using reverse genetics we exchanged the F genes of the AV324 and of a virulent NDV strain (Herts) and evaluated the recovered chimeric viruses for their pathogenicity in day-old chickens and in embryonated eggs. Our results show that the F protein of AV324, and most likely of similar other PPMV-1 strains, is functionally not different from that of virulent NDV strains and that the difference in pathogenicity must be determined by other factors.

Received 9 June 2009; accepted 29 July 2009.