| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Departments of Biology and Pathology, McMaster University, Hamilton, Ontario, Canada
Divisions of Virology and Biochemistry, National Institute for Medical Research, Mill Hill, London, NW7 1AA, U.K.
Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.
Research Student of the National Cancer Institute of Canada.
Present address: Department of Microbiology and Immunology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York, N.Y. 10461, USA.
Received 25 November 1976;
accepted 10 February 1977.
This article has been cited by other articles:
|
|
 |
|
  M. Arita, T. Wakita, and H. Shimizu Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity J. Gen. Virol., October 1, 2008; 89(10): 2518 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. K. Kang, R. Schiff, L. Ko, T. Wang, S. Y. Tsai, M.-J. Tsai, and B. W. O'Malley Dual Roles for Coactivator Activator and its Counterbalancing Isoform Coactivator Modulator in Human Kidney Cell Tumorigenesis Cancer Res., October 1, 2008; 68(19): 7887 - 7896. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ben-Israel, R. Sharf, G. Rechavi, and T. Kleinberger Adenovirus E4orf4 Protein Downregulates MYC Expression through Interaction with the PP2A-B55 Subunit J. Virol., October 1, 2008; 82(19): 9381 - 9388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Christensen, S. A. Byrd, A. K. Walker, J. R. Strahler, P. C. Andrews, and M. J. Imperiale Presence of the Adenovirus IVa2 Protein at a Single Vertex of the Mature Virion J. Virol., September 15, 2008; 82(18): 9086 - 9093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Keedy, W. Wang, J. Schiller, S. Chada, B. Slovis, K. Coffee, J. Worrell, L. A. Thet, D. H. Johnson, and D. P. Carbone Phase I Study of Adenovirus p53 Administered by Bronchoalveolar Lavage in Patients With Bronchioloalveolar Cell Lung Carcinoma: ECOG 6597 J. Clin. Oncol., September 1, 2008; 26(25): 4166 - 4171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Pich, S. Humme, M.-P. Spindler, A. Schepers, and W. Hammerschmidt Conditional gene vectors regulated in cis Nucleic Acids Res., August 1, 2008; 36(13): e83 - e83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Dube, K. L. Schornberg, T. S. Stantchev, M. I. Bonaparte, S. E. Delos, A. H. Bouton, C. C. Broder, and J. M. White Cell Adhesion Promotes Ebola Virus Envelope Glycoprotein-Mediated Binding and Infection J. Virol., July 15, 2008; 82(14): 7238 - 7242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Santarelli, A. Farina, M. Granato, R. Gonnella, S. Raffa, L. Leone, R. Bei, A. Modesti, L. Frati, M. R. Torrisi, et al. Identification and Characterization of the Product Encoded by ORF69 of Kaposi's Sarcoma-Associated Herpesvirus J. Virol., May 1, 2008; 82(9): 4562 - 4572. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Linden, V. R. Martins, M. A. M. Prado, M. Cammarota, I. Izquierdo, and R. R. Brentani Physiology of the Prion Protein Physiol Rev, April 1, 2008; 88(2): 673 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. W. Huang, W. Bi, G. N. Jenkins, and J. L. Alcorn Glucocorticoid Regulation of Human Pulmonary Surfactant Protein-B mRNA Stability Involves the 3'-Untranslated Region Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 473 - 482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Arita, Y. Ami, T. Wakita, and H. Shimizu Cooperative Effect of the Attenuation Determinants Derived from Poliovirus Sabin 1 Strain Is Essential for Attenuation of Enterovirus 71 in the NOD/SCID Mouse Infection Model J. Virol., February 15, 2008; 82(4): 1787 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Viita, J. Markkanen, E. Eriksson, M. Nurminen, K. Kinnunen, M. Babu, T. Heikura, S. Turpeinen, S. Laidinen, T. Takalo, et al. 15-Lipoxygenase-1 Prevents Vascular Endothelial Growth Factor A- and Placental Growth Factor-Induced Angiogenic Effects in Rabbit Skeletal Muscles via Reduction in Growth Factor mRNA Levels, NO Bioactivity, and Downregulation of VEGF Receptor 2 Expression Circ. Res., February 1, 2008; 102(2): 177 - 184. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. T. Aggarwal, Y. Chawengsub, K. M. Gauthier, H. Viita, S. Yla-Herttuala, and W. B. Campbell Endothelial 15-Lipoxygenase-1 Overexpression Increases Acetylcholine-Induced Hypotension and Vasorelaxation in Rabbits Hypertension, February 1, 2008; 51(2): 246 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Tao, J. Fujimoto, T. Men, X. Ye, J. Deng, L. Lacroix, J. L. Clifford, L. Mao, C. S. Van Pelt, J. J. Lee, et al. Identification of the Retinoic Acid Inducible Gprc5a As a New Lung Tumor Suppressor Gene J Natl Cancer Inst, November 21, 2007; 99(22): 1668 - 1682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Ewing, S. A. Byrd, J. B. Christensen, R. E. Tyler, and M. J. Imperiale Ternary Complex Formation on the Adenovirus Packaging Sequence by the IVa2 and L4 22-Kilodalton Proteins J. Virol., November 15, 2007; 81(22): 12450 - 12457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Sumner-Jones, D. R. Gill, and S. C. Hyde Lack of Repeat Transduction by Recombinant Adeno-Associated Virus Type 5/5 Vectors in the Mouse Airway J. Virol., November 15, 2007; 81(22): 12360 - 12367. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Armelini, K. M. Lima-Bessa, M. C. N. Marchetto, A. R. Muotri, V. Chigancas, R. A. Leite, H. Carvalho, and C. F. M. Menck Exploring DNA damage responses in human cells with recombinant adenoviral vectors Human and Experimental Toxicology, November 1, 2007; 26(11): 899 - 906. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Quigley, X. Huang, and Y. Yang Extent of Stimulation Controls the Formation of Memory CD8 T Cells J. Immunol., November 1, 2007; 179(9): 5768 - 5777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. L. Cianciola, D. Crooks, A. H. Shah, and C. Carlin A Tyrosine-Based Signal Plays a Critical Role in the Targeting and Function of Adenovirus RID{alpha} Protein J. Virol., October 1, 2007; 81(19): 10437 - 10450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Capper, B. Britt-Compton, M. Tankimanova, J. Rowson, B. Letsolo, S. Man, M. Haughton, and D. M. Baird The nature of telomere fusion and a definition of the critical telomere length in human cells Genes & Dev., October 1, 2007; 21(19): 2495 - 2508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zhang, N. Liu, S.-M. Park, Y. Wang, S. Byrne, A. E. Murmann, S. Bahr, M. E. Peter, S. T. Olson, A. Belaaouaj, et al. Serine Protease Inhibitor 6-Deficient Mice Have Increased Neutrophil Immunity to Pseudomonas aeruginosa J. Immunol., October 1, 2007; 179(7): 4390 - 4396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Parrott and M. B. Mathews Novel rapidly evolving hominid RNAs bind nuclear factor 90 and display tissue-restricted distribution Nucleic Acids Res., September 25, 2007; 35(18): 6249 - 6258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kuwata, R. Byrum, S. Whitted, R. Goeken, A. Buckler-White, R. Plishka, R. Iyengar, and V. M. Hirsch A Rapid Progressor-Specific Variant Clone of Simian Immunodeficiency Virus Replicates Efficiently In Vivo Only in the Absence of Immune Reponses J. Virol., September 1, 2007; 81(17): 8891 - 8904. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-i. Sakuragi, S. Sakuragi, and T. Shioda Minimal Region Sufficient for Genome Dimerization in the Human Immunodeficiency Virus Type 1 Virion and Its Potential Roles in the Early Stages of Viral Replication J. Virol., August 1, 2007; 81(15): 7985 - 7992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Nguyen, R. M. Kraft, and J. A. Blaho Susceptibility of cancer cells to herpes simplex virus-dependent apoptosis J. Gen. Virol., July 1, 2007; 88(7): 1866 - 1875. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Yea, J. Dembowy, L. Pacione, and M. Brown Microtubule-Mediated and Microtubule-Independent Transport of Adenovirus Type 5 in HEK293 Cells J. Virol., July 1, 2007; 81(13): 6899 - 6908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Moraes, T. de los Santos, M. Koster, T. Turecek, H. Wang, V. G. Andreyev, and M. J. Grubman Enhanced Antiviral Activity against Foot-and-Mouth Disease Virus by a Combination of Type I and II Porcine Interferons J. Virol., July 1, 2007; 81(13): 7124 - 7135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Baker, K. J. Rohleder, L. A. Hanakahi, and G. Ketner Adenovirus E4 34k and E1b 55k Oncoproteins Target Host DNA Ligase IV for Proteasomal Degradation J. Virol., July 1, 2007; 81(13): 7034 - 7040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M Smyth, S. L Ginn, C. T Deakin, G. J Logan, and I. E Alexander Limiting {gamma}c expression differentially affects signaling via the interleukin (IL)-7 and IL-15 receptors Blood, July 1, 2007; 110(1): 91 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Gill, J. L. Kutok, and J. D. Fingeroth Epstein-Barr Virus Thymidine Kinase Is a Centrosomal Resident Precisely Localized to the Periphery of Centrioles J. Virol., June 15, 2007; 81(12): 6523 - 6535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. D. Prickett and D. L. Brautigan Cytokine Activation of p38 Mitogen-Activated Protein Kinase and Apoptosis Is Opposed by alpha-4 Targeting of Protein Phosphatase 2A for Site-Specific Dephosphorylation of MEK3 Mol. Cell. Biol., June 15, 2007; 27(12): 4217 - 4227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Rechsteiner, C. Berger, M. Weber, J. A. Sigrist, D. Nadal, and M. Bernasconi Silencing of latent membrane protein 2B reduces susceptibility to activation of lytic Epstein-Barr virus in Burkitt's lymphoma Akata cells J. Gen. Virol., May 1, 2007; 88(5): 1454 - 1459. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Braren, S. Blank, H. Seismann, S. Deckers, M. Ollert, T. Grunwald, and E. Spillner Generation of Human Monoclonal Allergen-Specific IgE and IgG Antibodies from Synthetic Antibody Libraries Clin. Chem., May 1, 2007; 53(5): 837 - 844. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, X. Huang, and Y. Yang Innate Immune Response to Adenoviral Vectors Is Mediated by both Toll-Like Receptor-Dependent and -Independent Pathways J. Virol., April 1, 2007; 81(7): 3170 - 3180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Tyler, S. G. Ewing, and M. J. Imperiale Formation of a Multiple Protein Complex on the Adenovirus Packaging Sequence by the IVa2 Protein J. Virol., April 1, 2007; 81(7): 3447 - 3454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhu, X. Huang, and Y. Yang Type I IFN Signaling on Both B and CD4 T Cells Is Required for Protective Antibody Response to Adenovirus J. Immunol., March 15, 2007; 178(6): 3505 - 3510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-C. Huang, C.-M. Lee, M. Chen, M.-Y. Chung, Y.-H. Chang, W. J.-S. Huang, D. M.-T. Ho, C.-C. Pan, T. T. Wu, S. Yang, et al. Haplotypes, Loss of Heterozygosity, and Expression Levels of Glycine N-Methyltransferase in Prostate Cancer Clin. Cancer Res., March 1, 2007; 13(5): 1412 - 1420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Santosuosso, S. McCormick, E. Roediger, X. Zhang, A. Zganiacz, B. D. Lichty, and Z. Xing Mucosal Luminal Manipulation of T Cell Geography Switches on Protective Efficacy by Otherwise Ineffective Parenteral Genetic Immunization J. Immunol., February 15, 2007; 178(4): 2387 - 2395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Moise, M. Golczak, Y. Imanishi, and K. Palczewski Topology and Membrane Association of Lecithin: Retinol Acyltransferase J. Biol. Chem., January 19, 2007; 282(3): 2081 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Woo and A. J. Berk Adenovirus Ubiquitin-Protein Ligase Stimulates Viral Late mRNA Nuclear Export J. Virol., January 15, 2007; 81(2): 575 - 587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sherwood, H.-G. Burgert, Y.-H. Chen, S. Sanghera, S. Katafigiotis, R. E. Randall, I. Connerton, and K. H. Mellits Improved growth of enteric adenovirus type 40 in a modified cell line that can no longer respond to interferon stimulation J. Gen. Virol., January 1, 2007; 88(1): 71 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Xing and S. K. Tikoo E1A promoter of bovine adenovirus type 3 J. Gen. Virol., December 1, 2006; 87(12): 3539 - 3544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Trivedi, S. C. Zhang, A. B. Castoreno, W. Stockinger, E. C. Shieh, J. M. Vyas, E.-M. Frickel, and A. Nohturfft Immunoglobulin G signaling activates lysosome/phagosome docking PNAS, November 28, 2006; 103(48): 18226 - 18231. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Li, R. Santockyte, R.-F. Shen, E. Tekle, G. Wang, D. C. H. Yang, and P. B. Chock Expression of SUMO-2/3 Induced Senescence through p53- and pRB-mediated Pathways J. Biol. Chem., November 24, 2006; 281(47): 36221 - 36227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Grabusic, S. Maier, A. Hartmann, A. Mantik, W. Hammerschmidt, and B. Kempkes The CR4 region of EBNA2 confers viability of Epstein-Barr virus-transformed B cells by CBF1-independent signalling. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3169 - 3176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-P. Shih, C.-Y. Chen, S.-J. Liu, K.-H. Chen, Y.-M. Lee, Y.-C. Chao, and Y.-M. A. Chen Identifying Epitopes Responsible for Neutralizing Antibody and DC-SIGN Binding on the Spike Glycoprotein of the Severe Acute Respiratory Syndrome Coronavirus. J. Virol., November 1, 2006; 80(21): 10315 - 10324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-Y. Li, M.-Y. Liu, H.-M. Shih, C.-H. Tsai, and J.-Y. Chen Human cellular protein VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and enhances cell survival. J. Gen. Virol., October 1, 2006; 87(Pt 10): 2869 - 2878. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Prevost, M. O. Lonchampt, S. Holbeck, S. Attoub, D. Zaharevitz, M. Alley, J. Wright, M. C. Brezak, H. Coulomb, A. Savola, et al. Anticancer Activity of BIM-46174, a New Inhibitor of the Heterotrimeric G{alpha}/G{beta}{gamma} Protein Complex. Cancer Res., September 15, 2006; 66(18): 9227 - 9234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Fischer, S. Muller-Weeks, and S. J. Caradonna Fluorodeoxyuridine Modulates Cellular Expression of the DNA Base Excision Repair Enzyme Uracil-DNA Glycosylase. Cancer Res., September 1, 2006; 66(17): 8829 - 8837. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Warren, A. Rutkowski, and L. Cassimeris Infection with Replication-deficient Adenovirus Induces Changes in the Dynamic Instability of Host Cell Microtubules Mol. Biol. Cell, August 1, 2006; 17(8): 3557 - 3568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Renckens, J. J. T. H. Roelofs, S. Florquin, A. F. de Vos, J. M. Pater, H. R. Lijnen, P. Carmeliet, C. van 't Veer, and T. van der Poll Endogenous Tissue-Type Plasminogen Activator Is Protective during Escherichia coli-Induced Abdominal Sepsis in Mice J. Immunol., July 15, 2006; 177(2): 1189 - 1196. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Zschenker, T. Illies, and D. Ameis Overexpression of lysosomal Acid lipase and other proteins in atherosclerosis. J. Biochem., July 1, 2006; 140(1): 23 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Robert, N. Smadja-Lamere, M.-C. Landry, C. Champagne, R. Petrie, N. Lamarche-Vane, H. Hosoya, and J. N. Lavoie Adenovirus E4orf4 Hijacks Rho GTPase-dependent Actin Dynamics to Kill Cells: A Role for Endosome-associated Actin Assembly Mol. Biol. Cell, July 1, 2006; 17(7): 3329 - 3344. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Han and S. Swaminathan Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene ORF57 Is Essential for Infectious Virion Production. J. Virol., June 1, 2006; 80(11): 5251 - 5260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. McConnell, X. Danthinne, and M. J. Imperiale Characterization of a permissive epitope insertion site in adenovirus hexon. J. Virol., June 1, 2006; 80(11): 5361 - 5370. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hillgenberg, C. Hofmann, H. Stadler, and P. Loser High-efficiency system for the construction of adenovirus vectors and its application to the generation of representative adenovirus-based cDNA expression libraries. J. Virol., June 1, 2006; 80(11): 5435 - 5450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kubo, M. d. C. Seleme, H. S. Soifer, J. L. G. Perez, J. V. Moran, H. H. Kazazian Jr., and N. Kasahara L1 retrotransposition in nondividing and primary human somatic cells PNAS, May 23, 2006; 103(21): 8036 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Yoshikawa, T. Iwasaki, M. Ida-Hosonuma, M. Yoneyama, T. Fujita, H. Horie, M. Miyazawa, S. Abe, B. Simizu, and S. Koike Role of the Alpha/Beta Interferon Response in the Acquisition of Susceptibility to Poliovirus by Kidney Cells in Culture J. Virol., May 1, 2006; 80(9): 4313 - 4325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ohrmalm and G. Akusjarvi Cellular Splicing and Transcription Regulatory Protein p32 Represses Adenovirus Major Late Transcription and Causes Hyperphosphorylation of RNA Polymerase II. J. Virol., May 1, 2006; 80(10): 5010 - 5020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Neuhierl and H.-J. Delecluse The Epstein-Barr Virus BMRF1 Gene Is Essential for Lytic Virus Replication. J. Virol., May 1, 2006; 80(10): 5078 - 5081. [Abstract] [Full Text] [PDF]
|
|
