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Single amino acid changes in the Turnip mosaic virus viral genome-linked protein (VPg) confer virulence towards A. thaliana mutants knocked-out for eukaryotic initiation factors eIF(iso)4E and eIF(iso)4G

¹ INRA;
² CIRAD;
³ CBGP, UPM-INIA

⁴ E-mail: german{at}bordeaux.inra.fr

Previous resistance analyses of A. thaliana mutants knocked-out for eukaryotic translation initiation factors showed that disruption of either At-eIF(iso)4E or both At-eIF(iso)4G1 and At-eIF(iso)4G2 genes resulted in resistance against the Turnip mosaic virus (TuMV). In this study, we selected TuMV virulent variants overcoming those resistances and showed that two independent mutations in the region coding for the viral genome-linked protein (VPg) were sufficient to restore TuMV virulence in At-eif(iso)4e and At-eif(iso)4g1xAt-eif(iso)4g2 KO plants. As VPg-eIF(iso)4E interaction was previously shown to be critical for TuMV infection, a systematic analysis of the interactions between A. thaliana eIF4Es and VPgs of virulent and avirulent TuMV was performed. Our results suggest that the virulent TuMV variants could use an eIF4F-independent pathway.

Received 23 July 2009; accepted 9 September 2009.