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The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions

¹ University of Liège;
² University of Mons;
³ CERgroupe - Marloie;
⁴ VAR-CODA-CERVA - Ukkel

⁵ E-mail: a.vdplasschen{at}ulg.ac.be

Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the etiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome, and a tegument. In the present study, we identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry based proteomic approaches. In addition, exponentially modified protein abundance index (emPAI) analyses were used to estimate the relative abundance of identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins that were classified based on bioinformatic analyses as capsid (3), envelope (13), tegument (2) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated in CyHV-3 virions and the determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.

Received 17 July 2009; accepted 19 October 2009.