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Parvovirus PARV4 visualisation and detection

¹ NHS Blood and Transplant;
² Health Protection Agency, Centre for Infections

³ E-mail: hazel.appleton{at}hpa.org.uk

The parvovirus PARV4 is the most recently described member of the Parvoviridae which has a human host. To investigate the prevalence of PARV4 in the blood a quantitative TaqMan PCR (Q-PCR) was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high titre positive plasma had an approximate viral load of 5 x 10⁸ genome equivalents per ml. Two human sera identified as PARV4 antibody positive by indirect immunofluorescence were used in immune electron microscopy (IEM) to try and visualise native PARV4 virus within the high titre human plasma. PARV4 virus particles were observed using one of these two sera. To our knowledge this is the first time that native PARV4 virus has been visualised.

Received 10 July 2009; accepted 14 October 2009.