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Published online ahead of print on 5 August 2009 as doi:10.1099/vir.0.013946-0
Journal of General Virology 2009;90:2973.

A more recent version of this article appeared on December 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.013946-0
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Molecular differences between two Jeryl Lynn mumps virus vaccine component strains JL5 and JL2.

Philip Chambers, Bert K Rima1 and W Paul Duprex

Queen's University Belfast

1 E-mail: b.rima{at}qub.ac.uk

Summary The Jeryl Lynn (JL) vaccine against mumps virus (MuV) contains two components, MuVJL5 and MuVJL2, which differ by over 400 nucleotides. Due to the occurrence of bias in the direction of mutation, these differences and those found in nucleotide sequences of different isolates of the minor component in the vaccine (MuVJL2) might be due to the effect of ADAR like deaminases on MuV grown in tissue cultured cells. A molecular clone of MuVJL2 (pMuVJL2) and MuVJL2-specific helper plasmids were constructed in order to investigate molecular interactions between MuVJL5 and MuVJL2, to augment the existing molecular clone of MuVJL5 (pMuVJL5) and MuVJL5-specific helper plasmids (Clarke et al., 2000. Rescue of mumps virus from cDNA. J. Virol. 74:4831-4838). Genome and mRNA termini of MuV JL2 were characterized, and an unusual oligo-G insertion transcriptional editing event was detected near the F mRNA polyadenylation site of MuVJL2 but not of MuVJL5. Genes encoding glycoproteins of rMuVJL2 and rMuVJL5 have been exchanged to characterize the oligo-G insertion, which associated with the specific sequence of the F gene of MuVJL2 and with any other genes and the RNA dependent RNA polymerase of strain MuVJL2 .The results indicate that a single sequence G to A change obliterates the co-transcriptional editing of the F mRNA and that this oligo-G insertion does not affect the growth of the virus.

Received 9 June 2009; accepted 31 July 2009.




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