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Differential Binding Patterns to Host Cells Associated with Particles of Several Human Alpha Papillomavirus Types

Penn State College of Medicine

¹ E-mail: ndc1{at}psu.edu

The focus of this research was to compare the binding profiles of HPV11, 16, 18 and 45 VLPs to HaCaT cells and to the extracellular matrix (ECM) secreted by these cells. All four HPV types tested bind to a component(s) of the ECM. HPV11 VLP binding is blocked when the ECM is pretreated with an anti-Laminin 5 (LN5) Pab. A series of treatments utilizing heparins and heparinase reveals that HPV18 VLP is dependent on heparan sulfates (HS) for binding to cells and ECM. HPV16 and 45 VLP binding is dependent on HS for binding to HaCaT cells and on both HS and LN5 for binding to ECM. These studies emphasize the need to study the binding characteristics of different HPV types before applying universal binding principles to all papillomaviruses.

Received 21 April 2009; accepted 14 October 2009.