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Published online ahead of print on 2 September 2009 as doi:10.1099/vir.0.012112-0
Journal of General Virology 2009;90:3042.

A more recent version of this article appeared on December 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.012112-0
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Hibiscus chlorotic ringspot virus upregulates plant sulfite oxidase transcripts and increases sulfate levels in kenaf (Hibiscus cannabinus L.)

Xin Zhang and Sek-Man Wong1

National University of Singapore

1 E-mail: dbswsm{at}nus.edu.sg

Hibiscus chlorotic ringspot virus (HCRSV) coat protein (CP) is required for encapsidation and viral systemic movement. To better understand the roles of HCRSV CP in viral infection and its interactions with host proteins, a cDNA library of kenaf (Hibiscus cannabinus L.) was constructed and screened using a yeast two-hybrid system (YTHS) to identify CP-interacting proteins. One protein identified was sulfite oxidase (SO) and the interaction was confirmed in vitro and in vivo. The interaction was found to be associated with peroxisomes by immunofluorescent labeling of peroxisomes by anti-SKL signal peptide antibody. Our results showed that only the P and S domains of CP interacted with SO from kenaf (HcSO) by YTHS. This is probably due to the exposure of these two domains on the outer surface of the capsid. Peroxisomes were observed to aggregate in HCRSV infected cells, and biochemical assays of the total protein from kenaf leaf extracts showed that SO activity and SO-dependent H2O2-generating activity in the HCRSV-infected leaves increased compared to the mock-inoculated kenaf plants.

Received 23 March 2009; accepted 1 September 2009.




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