J Gen Virol
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Published online ahead of print on 1 July 2009 as doi:10.1099/vir.0.011213-0
Journal of General Virology 2009;90:2536.

A more recent version of this article appeared on October 1, 2009 J Gen Virol (2009), DOI 10.1099/vir.0.011213-0
© 2009 Society for General Microbiology
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Rapid screening of RNA silencing suppressors by using a recombinant virus derived from Beet necrotic yellow vein virus

Hubert Guilley, Diane Bortolamiol, Gérard Jonard, Salah Bouzoubaa and Véronique Ziegler-Graff1

Institut de Biologie Moléculaire des Plantes du CNRS, Strasbourg, France

1 E-mail: veronique.ziegler-graff{at}ibmp-ulp.u-strasbg.fr

To counteract plant defense mechanisms, plant viruses have evolved to encode RNA silencing suppressors proteins (RSS). These proteins can be identified by a range of silencing suppressors assays. Here, we describe a simple method using Beet necrotic yellow vein virus (BNYVV) that allows a rapid screening of RSS activity. The viral inoculum consists of BNYVV RNA1, which encodes proteins involved in viral replication, and two BNYVV-derived replicons: (1) rep3-P30, which expresses the movement protein P30 of Tobacco mosaic virus (TMV) and (2) rep5-X, which allows the expression of a putative RSS (X). This approach has been validated through the use of several known RSS. Two potential candidates have been tested and we show that, in our system, the P13 protein of Burdock mottle virus (BdMV) exhibits RSS activity while the P0 protein of Cereal yellow dwarf virus-RPV (CYDV-RPV) does not.

Received 23 February 2009; accepted 25 June 2009.


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A. Kozlowska-Makulska, H. Guilley, M. S. Szyndel, M. Beuve, O. Lemaire, E. Herrbach, and S. Bouzoubaa
P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity
J. Gen. Virol., April 1, 2010; 91(4): 1082 - 1091.
[Abstract] [Full Text] [PDF]


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