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Originally published as JGV in Press, 10.1099/vir.0.013276-0 on July 15, 2009 J Gen Virol 90 (2009), 2724-2730; DOI 10.1099/vir.0.013276-0

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Role of annexin A2 in cellular entry of rabbit vesivirus

Salomé González-Reyes, Alberto García-Manso, Gloria del Barrio{dagger}, Kevin P. Dalton, Lorenzo González-Molleda{ddagger}, José Arrojo-Fernández, Inés Nicieza and Francisco Parra

Departamento de Bioquímica y Biología Molecular, Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain

Correspondence
Francisco Parra
fparra{at}uniovi.es

The mechanisms of calicivirus attachment and internalization are not well understood, mainly due to the lack of a reliable cell-culture system for most of its members. In this study, rabbit vesivirus (RaV) virions were shown to bind annexin A2 (ANXA2) in a membrane protein fraction from HEK293T cells, using a virus overlay protein-binding assay and matrix-assisted laser desorption/ionization time-of-flight analysis. A monoclonal anti-ANXA2 antibody and small interfering RNA-mediated knockdown of ANXA2 expression in HEK293T cells reduced virus infection significantly, further supporting the role of ANXA2 in RaV attachment and/or internalization.

{dagger}Present address: Departamento de Microbiología y Virología, Facultad de Biología, Universidad de La Habana, Ciudad de La Habana, Cuba.

{ddagger}Present address: Department of Microbiology, University of Pennsylvania, School of Dental Medicine, 240 South 40th Street, Philadelphia, PA 19104, USA.

A supplementary figure showing characterization of the 33 kDa protein is available with the online version of this paper.






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