J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published as JGV in Press, 10.1099/vir.0.012724-0 on July 8, 2009 J Gen Virol 90 (2009), 2569-2574; DOI 10.1099/vir.0.012724-0

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary table
Right arrow All Versions of this Article:
vir.0.012724-0v1
90/10/2569    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Saunders, G. C.
Right arrow Articles by Lantier, F.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Saunders, G. C.
Right arrow Articles by Lantier, F.
Agricola
Right arrow Articles by Saunders, G. C.
Right arrow Articles by Lantier, F.

Short Communication

Protective effect of the T112 PrP variant in sheep challenged with bovine spongiform encephalopathy

G. C. Saunders1, I. Lantier2, S. Cawthraw1, P. Berthon2, S. J. Moore3, M. E. Arnold4, O. Windl1, M. M. Simmons3, O. Andréoletti5, S. Bellworthy3 and F. Lantier2

1 Molecular Pathogenesis and Genetics Department, Veterinary Laboratories Agency (VLA Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK
2 INRA, UR1282, Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France
3 Pathology Department, VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
4 CERA, VLA Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK
5 UMR INRA-ENVT, Interactions Hôtes–Agents Pathogènes, Ecole Vétérinaire de Toulouse, F-310761 Toulouse, France

Correspondence
S. Cawthraw
s.cawthraw{at}vla.defra.gsi.gov.uk

Sheep with an ARQ/ARQ PRNP genotype at codon positions 136/154/171 are highly susceptible to experimental infection with bovine spongiform encephalopathy (BSE). However, a number of sheep challenged orally or intracerebrally with BSE were clinically asymptomatic and found to survive or were diagnosed as BSE-negative when culled. Sequencing of the full PRNP gene open reading frame of BSE-susceptible and -resistant sheep indicated that, in the majority of Suffolk sheep, resistance was associated with an M112T PRNP variant (TARQ allele). A high proportion (47 of 49; 96 %) of BSE-challenged wild-type (MARQ/MARQ) Suffolk sheep were BSE-infected, whereas none of the 20 sheep with at least one TARQ allele succumbed to BSE. Thirteen TARQ-carrying sheep challenged with BSE are still alive and some have survival periods equivalent to, or greater than, reported incubation periods of BSE in ARR/ARR and VRQ/VRQ sheep.

A supplementary table detailing the ovine PRNP ORF PCR amplification conditions used is available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 by the Society for General Microbiology.