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Molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (PBV) strain: evidence for high genetic diversity with genogroup I PBVs

¹ Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan
² Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Tochigi-ken, Japan
³ School of Biology, National Institute of Science Education and Research, Bhubaneswar, India

Correspondence
S. Ghosh
souvikrota{at}gmail.com or
souvik8{at}rediffmail.com

We report here the molecular characterization of a bovine genogroup I picobirnavirus strain RUBV-P detected from a 1-month-old diarrhoeic calf in eastern India. Sequence comparisons and phylogenetic analysis of a short stretch of gene segment 2 of RUBV-P revealed low nucleotide identities (51.2–64.9 %) with and distant genetic relatedness to other genogroup I picobirnaviruses. The complete gene segment 2 sequence of RUBV-P was obtained by the single primer amplification method with modifications. Gene segment 2 of RUBV-P was 1758 bp long, encoded a predicted protein of 554 aa and exhibited low nucleotide (58.1–58.8 %) and amino acid (51.3–55.4 %) identities with genogroup I human strains Hy005102 and 1-CHN-97. The 5'- and 3'-end nucleotide sequences, and the three motifs of RNA-dependent RNA polymerases of double-stranded RNA viruses, were conserved among these strains. Our findings suggested that bovine strain RUBV-P might be distinct from genogroup I picobirnaviruses of humans and other animals.

The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is GQ221268.

A supplementary figure showing comparative analysis of the partial deduced amino acid sequence of gene segment 2 of bovine picobirnavirus strain RUBV-P with the cognate stretch of other genogroup I strains is available with the online version of this paper.