| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Virology Division
and2 Disease Assessment Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701-5011, U.S.A.
A panel of 24 monoclonal antibodies (MAbs) to the G1 or G2 envelope glycoproteins of Hantaan virus were used to determine the surface topography and functional properties of antigenic sites. Nine distinct, partially overlapping antigenic sites, two on G1 and seven on G2, were demonstrated by competitive binding assays. Analyses of the antigenic sites by haemagglutination (HA) inhibition and plaque-reduction neutralization tests showed that all of the sites, except one on G1, were related to viral HA. Only one of the G1 antigenic sites and two of the G2 sites were involved in virus neutralization. These results suggest that certain epitopes related to HA were not critical for virus neutralization. The nine antigenic sites could be further divided into 13 based upon the serological cross-reactivity of MAbs with viruses representative of each of the four known antigenic groups within the Hantavirus genus of Bunyaviridae, i.e. Hantaan, Seoul, Puumala and Prospect Hill viruses.
Keywords: Hantaan virus, HFRS, monoclonal antibodies
Present address: Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060, Japan.
Received 17 August 1988;
accepted 26 October 1988.
This article has been cited by other articles:
|
|
 |
|
  M. Okumura, K. Yoshimatsu, S. Kumperasart, I. Nakamura, M. Ogino, M. Taruishi, A. Sungdee, S. Pattamadilok, I. N. Ibrahim, S. Erlina, et al. Development of Serological Assays for Thottapalayam Virus, an Insectivore-Borne Hantavirus Clin. Vaccine Immunol., February 1, 2007; 14(2): 173 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. PATTAMADILOK, B.-H. LEE, S. KUMPERASART, K. YOSHIMATSU, M. OKUMURA, I. NAKAMURA, K. ARAKI, Y. KHOPRASERT, P. DANGSUPA, P. PANLAR, et al. GEOGRAPHICAL DISTRIBUTION OF HANTAVIRUSES IN THAILAND AND POTENTIAL HUMAN HEALTH SIGNIFICANCE OF THAILAND VIRUS Am J Trop Med Hyg, November 1, 2006; 75(5): 994 - 1002. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Yu, M. Liang, B. Fan, H. Xu, C. Li, Q. Zhang, D. Li, B. Tang, S. Li, Y. Dai, et al. Maternally Derived Recombinant Human Anti-Hantavirus Monoclonal Antibodies Are Transferred to Mouse Offspring during Lactation and Neutralize Virus In Vitro. J. Virol., April 1, 2006; 80(8): 4183 - 4186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ogino, K. Yoshimatsu, H. Ebihara, K. Araki, B.-H. Lee, M. Okumura, and J. Arikawa Cell Fusion Activities of Hantaan Virus Envelope Glycoproteins J. Virol., October 1, 2004; 78(19): 10776 - 10782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shi and R. M. Elliott Analysis of N-Linked Glycosylation of Hantaan Virus Glycoproteins and the Role of Oligosaccharide Side Chains in Protein Folding and Intracellular Trafficking J. Virol., May 15, 2004; 78(10): 5414 - 5422. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Custer, E. Thompson, C. S. Schmaljohn, T. G. Ksiazek, and J. W. Hooper Active and Passive Vaccination against Hantavirus Pulmonary Syndrome with Andes Virus M Genome Segment-Based DNA Vaccine J. Virol., September 15, 2003; 77(18): 9894 - 9905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Markotic, L. Hensley, T. Geisbert, K. Spik, and C. Schmaljohn Hantaviruses induce cytopathic effects and apoptosis in continuous human embryonic kidney cells J. Gen. Virol., August 1, 2003; 84(8): 2197 - 2202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Heiskanen, X.-D. Li, J. Hepojoki, E. Gustafsson, A. Lundkvist, A. Vaheri, and H. Lankinen Improvement of binding of Puumala virus neutralization site resembling peptide with a second-generation phage library Protein Eng. Des. Sel., June 1, 2003; 16(6): 443 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ogino, H. Ebihara, B.-H. Lee, K. Araki, A. Lundkvist, Y. Kawaoka, K. Yoshimatsu, and J. Arikawa Use of Vesicular Stomatitis Virus Pseudotypes Bearing Hantaan or Seoul Virus Envelope Proteins in a Rapid and Safe Neutralization Test Clin. Vaccine Immunol., January 1, 2003; 10(1): 154 - 160. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yoshimatsu, B.-H. Lee, K. Araki, M. Morimatsu, M. Ogino, H. Ebihara, and J. Arikawa The Multimerization of Hantavirus Nucleocapsid Protein Depends on Type-Specific Epitopes J. Virol., December 20, 2002; 77(2): 943 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. E. Severson, C. S. Schmaljohn, A. Javadian, and C. B. Jonsson Ribavirin Causes Error Catastrophe during Hantaan Virus Replication J. Virol., December 6, 2002; 77(1): 481 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-Y. Kim, Y. Choi, H.-S. Cheong, and J. Choe Identification of a cell surface 30 kDa protein as a candidate receptor for Hantaan virus J. Gen. Virol., April 1, 2002; 83(4): 767 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Hooper, D. M. Custer, E. Thompson, and C. S. Schmaljohn DNA Vaccination with the Hantaan Virus M Gene Protects Hamsters against Three of Four HFRS Hantaviruses and Elicits a High-Titer Neutralizing Antibody Response in Rhesus Monkeys J. Virol., September 15, 2001; 75(18): 8469 - 8477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. de Carvalho Nicacio, M. Sällberg, C. Hultgren, and A. Lundkvist T-helper and humoral responses to Puumala hantavirus nucleocapsid protein: identification of T-helper epitopes in a mouse model J. Gen. Virol., January 1, 2001; 82(1): 129 - 138. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ebihara, K. Yoshimatsu, M. Ogino, K. Araki, Y. Ami, H. Kariwa, I. Takashima, D. Li, and J. Arikawa Pathogenicity of Hantaan Virus in Newborn Mice: Genetic Reassortant Study Demonstrating that a Single Amino Acid Change in Glycoprotein G1 Is Related to Virulence J. Virol., October 1, 2000; 74(19): 9245 - 9255. [Abstract] [Full Text]
|
|
|
|
|
|
K Nemirov, O Vapalahti, A Lundkvist, V Vasilenko, I Golovljova, A Plyusnina, J Niemimaa, J Laakkonen, H Henttonen, A Vaheri, et al. Isolation and characterization of Dobrava hantavirus carried by the striped field mouse (Apodemus agrarius) in Estonia J. Gen. Virol., February 1, 1999; 80(2): 371 - 379. [Abstract]
|
|
|
|
 |
|
 I. Gavrilovskaya, R. LaMonica, M.-E. Fay, B. Hjelle, C. Schmaljohn, R. Shaw, and E. R. Mackow New York 1 and Sin Nombre Viruses Are Serotypically Distinct Viruses Associated with Hantavirus Pulmonary Syndrome J. Clin. Microbiol., January 1, 1999; 37(1): 122 - 126. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
