| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Viruskrankheiten der Pflanzen, Messeweg 11/12, D-3300 Braunschweig, F.R.G.
and2 Universität für Bodenkultur, Institut für Angewandte Mikrobiologie, Peter Jordan-Strasse 82, A-1190 Wien, Austria
The complete nucleotide sequence of the RNA of an aphid non-transmissible plum pox virus (PPV-NAT) isolate has been determined from five overlapping cDNA clones. cDNA prepared by primer extension was used to determine the 5' terminus. The assembled RNA is 9741 nucleotides in length, excluding a 3' terminal poly(A) sequence. One large open reading frame starts at nucleotide positions 36 to 38 and is terminated with an UAG codon at positions 9522 to 9524. The putative start codon is located at positions 147 to 149. The encoded polyprotein has a predicted Mr of 353.8K. Comparison of cistrons from tobacco vein mottling virus and tobacco etch virus with those predicted for PPV-NAT indicated a similar genome organization. A highly conserved sequence of 12 nucleotides was found in the 5' non-coding region of these three potyviruses. The potential polyadenylation signal from yeast (UAUGU) was found in the 3' non-coding region of PPV-NAT and several other members of the potyvirus group.
Keywords: plum pox virus, nucleotide sequence, genome organization
Received 5 September 1988;
accepted 18 November 1988.
This article has been cited by other articles:
|
|
 |
|
  M. Varrelmann, E. Maiss, R. Pilot, and L. Palkovics Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing J. Gen. Virol., March 1, 2007; 88(3): 1005 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Goytia, L. Fernandez-Calvino, B. Martinez-Garcia, D. Lopez-Abella, and J. J. Lopez-Moya Production of plum pox virus HC-Pro functionally active for aphid transmission in a transient-expression system. J. Gen. Virol., November 1, 2006; 87(Pt 11): 3413 - 3423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Waltermann and E. Maiss Detection of 6K1 as a mature protein of 6 kDa in plum pox virus-infected Nicotiana benthamiana. J. Gen. Virol., August 1, 2006; 87(Pt 8): 2381 - 2386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Dietrich and E. Maiss Fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected Nicotiana benthamiana plants J. Gen. Virol., October 1, 2003; 84(10): 2871 - 2876. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Spetz, A. M. Taboada, S. Darwich, J. Ramsell, L. F. Salazar, and J. P. T. Valkonen Molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in Peru J. Gen. Virol., September 1, 2003; 84(9): 2565 - 2578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Roudet-Tavert, S. German-Retana, T. Delaunay, B. Delecolle, T. Candresse, and O. Le Gall Interaction between potyvirus helper component-proteinase and capsid protein in infected plants J. Gen. Virol., June 1, 2002; 83(7): 1765 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-R. Choi, K. M. Horken, D. C. Stenger, and R. French Mapping of the P1 proteinase cleavage site in the polyprotein of Wheat streak mosaic virus (genus Tritimovirus) J. Gen. Virol., February 1, 2002; 83(2): 443 - 450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. N. Manoussopoulos, E. Maiss, and M. Tsagris Native electrophoresis and Western blot analysis (NEWeB): a method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues J. Gen. Virol., September 1, 2000; 81(9): 2295 - 2298. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Varrelmann, L. Palkovics, and E. Maiss Transgenic or Plant Expression Vector-Mediated Recombination of Plum Pox Virus J. Virol., August 15, 2000; 74(16): 7462 - 7469. [Abstract] [Full Text]
|
|
|
|
|
|
P. Sáenz, M. T. Cervera, S. Dallot, L. Quiot, J.-B. Quiot, J. L. Riechmann, and J. A. García Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K1 J. Gen. Virol., March 1, 2000; 81(3): 557 - 566. [Abstract] [Full Text]
|
|
|
|
|
|
M. Varrelmann and E. Maiss Mutations in the coat protein gene of Plum pox virus suppress particle assembly, heterologous encapsidation and complementation in transgenic plants of Nicotiana benthamiana J. Gen. Virol., March 1, 2000; 81(3): 567 - 576. [Abstract] [Full Text]
|
|
|
|
|
|
J. J. López-Moya, R. Y. Wang, and T. P. Pirone Context of the coat protein DAG motif affects potyvirus transmissibility by aphids J. Gen. Virol., December 1, 1999; 80(12): 3281 - 3288. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
