HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Devic, M. Articles by Baulcombe, D. Search for Related Content PubMed PubMed Citation Articles by Devic, M. Articles by Baulcombe, D. Agricola Articles by Devic, M. Articles by Baulcombe, D.

Symptom Production on Tobacco and Tomato Is Determined by Two Distinct Domains of the Satellite RNA of Cucumber Mosaic Virus (Strain Y)

¹ The Sainsbury Laboratory, John Innes Institute, Colney Lane, Norwich NR4 7UH
and² Department of Molecular Genetics, Institute of Plant Science Research, Maris Lane, Trumpington, Cambridge CB2 2JB, U.K.

Complementary DNAs of two different satellite RNA isolates from cucumber mosaic virus (CMV), Y from Japan and Ra from France, have been cloned in a transcription vector containing the Pr promoter. When inoculated on plants with CMV RNA (strain KIN), the transcripts of the cloned Y satellite cDNA elicit a bright yellow mosaic on tobacco and a lethal necrosis on tomato. Addition of the transcripts of the Ra satellite cDNA to an inoculum of CMV RNA resulted in symptom attenuation on both tobacco and tomato, in agreement with the characterized symptoms of the natural satellite. Recombinant molecules involving these two satellites have been constructed in order to determine which parts of the Y satellite RNA are involved in symptom induction. The determinant for symptom production on tobacco lies in the region between nucleotides 1 and 219. The domain for necrotic symptoms on tomato resides on the 3' half of the molecule beyond nucleotide 219.

Keywords: CMV satellite RNA, tomato, tobacco

Received 14 March 1989; accepted 8 June 1989.

This article has been cited by other articles:

				D.C. BAULCOMBE Short Silencing RNA: The Dark Matter of Genetics? Cold Spring Harb Symp Quant Biol, January 1, 2006; 71(0): 13 - 20. [Abstract] [PDF]

				N. Yamaguchi, Y. Seshimo, E. Yoshimoto, H. I. Ahn, K. H. Ryu, J. K. Choi, and C. Masuta Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA J. Gen. Virol., August 1, 2005; 86(8): 2359 - 2369. [Abstract] [Full Text] [PDF]