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1 National Agriculture Research Center
and2 National Institute of Agrobiological Resources, Tsukuba Science City, Yatabe, Ibaraki 305, Japan
The nucleotide sequence of DNA complementary to the eighth largest (S8) of the 12 genome segments of rice dwarf virus was determined. This genome segment is 1424 nucleotides in length and has a single long open reading frame extending 1260 nucleotides from the first AUG triplet (residues 24 to 26). The predicted translational product comprises 420 amino acids and has an Mr of 46422. The amino acid sequences of several peptide fragments of the major outer capsid protein were found to be contained in the predicted translational product of the above nucleotide sequence. This protein, previously reported to be 43K, is encoded by genome segment S8 and therefore renamed the 46K protein.
Keywords: rice dwarf virus, outer capsid protein, nucleotide sequence
Present address: University of Tokyo, Bunkyo-Ku, Tokyo 113, Japan.
Received 29 March 1989;
accepted 14 June 1989.
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