HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Anderson, D. L. Articles by Gibbs, A. J. Search for Related Content PubMed Articles by Anderson, D. L. Articles by Gibbs, A. J. Agricola Articles by Anderson, D. L. Articles by Gibbs, A. J.

Inapparent Virus Infections and their Interactions in Pupae of the Honey Bee (Apis mellifera Linnaeus) in Australia

D. L. Anderson

Research School of Biological Sciences, Australian National University, Canberra 2601, Australia

When honey bee pupae from seemingly healthy Australian colonies were injected with various salt solutions, inapparent infections of black queen-cell virus (BQCV), Kashmir bee virus (KBV), sacbrood virus (SBV) and, occasionally, cricket paralysis virus were activated. The activated viruses replicated to detectable concentrations after pupae were incubated at 35 °C for 3 days. Inapparent infections of SBV, but not the other viruses, were also activated merely by incubating pupae at 35 °C. The replication of activated BQCV, KBV or SBV was specifically suppressed by injecting pupae with rabbit antisera against the particles of each virus. However, a greater proportion of activated SBV infections occurred in groups of pupae in which the replication of activated KBV was suppressed than in groups of pupae in which KBV was not suppressed. In addition, a greater proportion of activated BQCV infections occurred in pupae in which the replication of both KBV and SBV was suppressed than in groups of pupae that were not injected with KBV and SBV antisera. Results from one group of pupae treated in this way indicated that about 40% were infected with KBV, about 43% with SBV and about 15% with BQCV. There was no evidence of interference between the viruses in the establishment of inapparent infections of pupae, but clear evidence of interference during or after activation. These results have implications for the use of pupae for virus propagation.

Keywords: honey bee viruses, inapparent infection, virus activation

Present address: Division of Scientific and Industrial Research, Entomology Division, Mount Albert Research Centre, Private Bag, Auckland, New Zealand.

Received 23 November 1987; accepted 28 March 1988.

This article has been cited by other articles:

				E. Maori, S. Lavi, R. Mozes-Koch, Y. Gantman, Y. Peretz, O. Edelbaum, E. Tanne, and I. Sela Isolation and characterization of Israeli acute paralysis virus, a dicistrovirus affecting honeybees in Israel: evidence for diversity due to intra- and inter-species recombination J. Gen. Virol., December 1, 2007; 88(12): 3428 - 3438. [Abstract] [Full Text] [PDF]

				G. Lanzi, J. R. de Miranda, M. B. Boniotti, C. E. Cameron, A. Lavazza, L. Capucci, S. M. Camazine, and C. Rossi Molecular and Biological Characterization of Deformed Wing Virus of Honeybees (Apis mellifera L.). J. Virol., May 1, 2006; 80(10): 4998 - 5009. [Abstract] [Full Text] [PDF]