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1 Department of Tumour Biology, Karolinska Institute, Box 60400, 10401 Stockholm, Sweden
2 E.I. Du Pont de Nemours & Co., Biomedical Products Department, Billerica, Massachusetts 01862
3 New England Regional Primate Research Center, Southborough, Massachusetts 01772
and The4 Joseph Stokes Jr Research Institute, The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, U.S.A.
Nuclear DNA-binding proteins were extracted from lymphoblastoid cell lines transformed with Epstein-Barr virus (EBV) or with the related lymphotropic herpesviruses of gorilla (Herpesvirus gorilla), chimpanzee (H. pan), baboon (H. papio) or orang-utan (H. pongo). They were immunoblotted with the sera of all four simian species in comparison with EBV antibody-positive human sera. Eight nuclear proteins were identified, and were designated GONA-1 and GONA-2 for H. gorilla-determined nuclear antigens, PANA-1A, PANA-1B and PANA-2 for H. pan, PONA-2 for H. pongo and HUPNA-1 and HUPNA-2 for H. papio-determined nuclear antigens. One of two tested HUPNA-2-positive baboon sera and one PONA-2-positive orang-utan serum also reacted with EBNA-2 in EBV-transformed cells. A human serum that contained antibodies to all five EBNA proteins cross-reacted only with PANA-2 and PONA-2. Monospecific anti-peptide antibodies against EBNA-2, type A, also reacted with PONA-2, but not with the other simian nuclear antigens. The data provide evidence that EBV-like simian lymphotropic herpesviruses induce EBNA-like nuclear antigens and that EBNA-2 and some simian EBNA-related proteins contain an epitope that has been conserved during the evolution of the EBV family of viruses.
Keywords: herpesvirus, simian, nuclear proteins, EBV
Present address: Department of Molecular Biology, Scripps Clinic and Research Foundation, 10666 North Torrey Pines Road, La Jolla, California 92037, U.S.A.
Received 20 November 1986;
accepted 12 February 1987.
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