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Isolation of Two Bacteriophages from Bacillus larvae, PBL1 and PBL0.5, and Partial Characterization of PBL1

Douglas W. Dingman

Christopher C. Field

Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, U.S.A.

Two temperate bacteriophages have been isolated from Bacillus larvae: PBL1 and PBL0.5. Strains lysogenic for either of these phages are immune to lysis by the same phage but are sensitive to the other phage. PBL1 has an oval head, a non-contractile tail, and a base plate with a pin structure but no apparent tail fibres. The genome of PBL1 consists of double-stranded DNA with a molecular weight of 24.1 (±0.6) x 10⁶, a G + C content (derived from melting temperature) of 41.5%, and cohesive ends. Restriction enzyme analysis permitted construction of a physical map of the genome.

Keywords: phages, Bacillus larvae, characterization

Present address: Department of Molecular Biology and Microbiology, School of Medicine, Tufts University, Boston, Massachusetts 02111, U.S.A.

Present address: Department of Life Sciences, Southwest Missouri State University, Springfield, Missouri 65802, U.S.A.

Received 20 January 1984; accepted 20 February 1984.