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Baculovirus Bioassay Not Dependent Upon Polyhedra Production

Department of Entomological Sciences University of California, Berkeley California 94720, U.S.A.

An immunoperoxidase-based quantitative assay has been developed for infectious units of Autographa californica nuclear polyhedrosis virus infecting TN-368 cells. Major benefits of this assay over existing quantitative assays for baculoviruses are that it is not dependent upon polyhedra production, it is clear-cut and easy to score, and results are obtained within 24 h.

Keywords: baculovirus, in vitro assay, immunoperoxidase staining

Received 23 February 1981; accepted 7 May 1981.

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