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J Gen Virol 53 (1981), 363-369; DOI 10.1099/0022-1317-53-2-363
© 1981 Society for General Microbiology
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Protein Synthesis in Cells Infected with Bovine Rotavirus

V. Urquidi, E. Novo and J. Esparza{dagger}

Laboratorio de Biología de Virus Instituto Venezolano de Investigaciones, Científicas Apartado 1827, Caracas 1010A, Venezuela

Bovine rotavirus was found to multiply efficiently in LLC-MK2 cells, a continuous line of rhesus monkey kidney, with a growth cycle which was essentially completed within 9 h after infection. The presence of low concentrations of trypsin (10 µg/ml) in the virus inoculum was essential for infectivity. Polyacrylamide gel electrophoresis of infected cell extracts demonstrated the synthesis of at least eight virus-specific polypeptides 6 h post-infection with mol. wt. ranging from 102 x 103 to 29 x 103. Six polypeptides (about p102K, p91K, p84K, p45K, p37K and p34K) were identified as structural components of the virion. Two other polypeptides (p54K and p29K) were identified as non-structural components. The synthesis of non-structural polypeptides appeared to precede that of the structural proteins. Pulse-chase experiments showed only one minor post-translation modification of the virus-specified proteins, namely an increase in the mobility of the 29K polypeptide.

{dagger} Present address: Department of Microbiology and Immunology, Duke University Medical Center, Box 3020, Durham, North Carolina 27710, U.S.A.

Received 8 September 1980; accepted 8 December 1980.




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Copyright © 1981 by the Society for General Microbiology.