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Interference and Neutralization Studies with BRYAN Strain Rous Sarcoma Virus Synthesized in the Absence of Helper Virus

Department of Anatomy and Embryology, University College London, Gower Street, London, W.C.1

The relationship of RSV(O) to other avian tumour viruses was studied by virus interference and serological cross neutralization. Viruses of subgroup B interfere with RSV(O) on chicken cells but not on quail cells. Viruses of sub-groups A and C enhance the focus forming efficiency of RSV(O). Antisera to RSV(O) and avian tumour viruses of sub-group A, B and C do not cross-react, but serological cross-reaction was found between RSV(O) and sub-group D.

The ability of RSV(O) and L-R cells to stimulate anti-viral antibodies has been investigated.

No helper virus in RSV(O) stocks was demonstrable by interference assays.

Received 29 May 1969; accepted 20 July 1969.

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