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The Effect of Polyamines on Herpes Simplex Virus Type 1 DNA Polymerase Purified from Infected Baby Hamster Kidney Cells (BHK-21/C13)

Department of Biochemistry University of Aberdeen Marischal College, Aberdeen AB9 1AS, U.K.

The DNA polymerase whose synthesis is directed by the herpes simplex virus type 1 (HSV-1) DNA was purified 545-fold from BHK-21/C13 cells 16 h after infection with the virus. Spermidine and spermine stimulated the activity of the polymerase over the concentration range 0.5 mM to 2.5 mM. This effect was enhanced with increased concentrations of polyamine, maximum stimulation being threefold and fourfold for spermidine and spermine respectively. The diamine, putrescine, had little effect on the enzyme at the concentrations used (0.25 to 1.25 mM).

Received 3 December 1979; accepted 7 February 1980.