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The Characteristics of the Cell-free Translation of mRNA from Cells Infected with the Herpes Virus Pseudorabies Virus

Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.

The translation in vitro of mRNA from pseudorabies virus infected cells was studied using systems derived from wheat germ and from rabbit reticulocyte. The mRNA was shown by molecular hybridization to contain sequences complementary to virus DNA. Products of in vitro translation co-migrating with virus proteins on polyacrylamide gel electropherograms were detected and the major capsid protein (mol. wt. 150000) was shown to be present among the products by immune precipitation. Optimum conditions for the stimulation of amino acid incorporation in vitro were determined and found to be similar for mRNA from both infected and mock-infected cells.

^* Present address: Institute of Virology, University of Glasgow, Glasgow G12 8QQ, Scotland, U.K.

Received 17 December 1979; accepted 5 March 1980.