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Typing Mycobacterium tuberculosis with Mycobacteriophage Bo4

Mycobacteriology Branch, Bacteriology Division, Bureau of Laboratories, Center for Disease Control, Public Health Service, U.S. Department of Health, Education and Welfare, Atlanta, Georgia 30333, U.S.A.

Mycobacteriophage B04 grown on the indicator host strain Mycobacterium fortuitum SN203 was restricted and modified by Mycobacterium tuberculosis H₃₇R_v. Phage B04·H₃₇R_v was restricted and modified by the alternate host SN 203. M. tuberculosis strain Myc 1025 was described as a r^-m^- isolate. By using the mycobacterial prototype strains for phage typing wild M. tuberculosis isolates, it was demonstrated that only the modified phage B04·H₃₇R_v was a potential typing phage.

Received 5 October 1979; accepted 5 March 1980.