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Naval Biosciences Laboratory, and Department of Biomedical and Environmental Health Sciences, University of California, School of Public Health, Berkeley, Calif. 94720, U.S.A.
Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with 125I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled with 32P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15000.
* Present address: Department of Neurology, University of California Medical Center, San Francisco, Calif. 94143, U.S.A.
Present address: Reed Neurological Research Center, University of California, Los Angeles, Calif. 90024, U.S.A.
Received 29 January 1979;
accepted 22 October 1979.
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