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Polyethylene Glycol-mediated Infection of Cucumber Protoplasts by Cucumber Mosaic Virus and Virus RNA

Department of Microbiology, University of Birmingham, Birmingham B15 2TT, England

In attempts to infect cucumber protoplasts with cucumber mosaic virus (CMV) RNA, the use of polyethylene glycol as an uptake inducer proved successful. This method involves resuspending protoplasts in high concentrations of polyethylene glycol in the presence of RNA, followed by rapid dilution. Levels of infection obtained with CMV-RNA were more reproducible than those using virus nucleoprotein. This method was also suitable for the inoculation of protoplasts with tobacco ringspot virus RNA.

Received 17 July 1979; accepted 12 October 1979.

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