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Interferon Production by Individual L Cells

Edward H. Simon

Department of Biological Sciences, Purdue University, W. Lafayette, Indiana 47907, U.S.A.

Using a protected centre technique in which agarose prevents the diffusion of interferon from individual producing cells, we have shown that essentially every cell in a monolayer of mouse L cells can be induced to produce interferon by infection with Newcastle disease virus (NDV). The amount of interferon produced by individual cells appeared to be highly variable, even when cloned cells and viruses were used. U.v.-irradiated virus lost its capacity to induce interferon in L cells and to infect chick embryo fibroblasts at the same rate. A small proportion of cells (1 x 10^-6 to 10 x 10^-6) appeared to produce interferon constitutively. This fraction was increased threefold by u.v. irradiation of the cells, and up to 10-fold by exposing cells to the mutagen ethyl methane sulphonate.

^* Present address: National Institutes of Health, Baltimore Cancer Research Center, 3100 Wyman Park Drive, Baltimore, Md. 21211.

To whom reprint requests should be addressed.

Received 1 May 1979; accepted 10 October 1979.