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J Gen Virol 44 (1979), 565-568; DOI 10.1099/0022-1317-44-2-565
© 1979 Society for General Microbiology
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Efficient Mechanical Inoculation of Turnip Yellow Mosaic Virus Using Small Volumes of Inoculum

L. Fraser and R. E. F. Matthews

Department of Cell Biology University of Auckland, Private Bag Auckland, New Zealand

When purified turnip yellow mosaic virus was inoculated mechanically on to Chinese cabbage leaves, using known numbers of virus particles in 0.1 to 1.0 µl volumes of inoculum, as few as 10 to 30 particles were required to produce a single local lesion. A simple procedure is described for obtaining local lesions from the virus in single infected protoplasts.

Received 7 February 1979; accepted 23 March 1979.




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Copyright © 1979 by the Society for General Microbiology.