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In Vitro Synthesis of Cowpea Chlorotic Mottle Virus Polypeptides

Departments of Molecular Biology and Virology Agricultural University, Wageningen, The Netherlands

The ribonucleic acids of cowpea chlorotic mottle virus were separated by rate zonal centrifugation, followed by polyacrylamide gel electrophoresis. They were then translated in wheat germ extracts and rabbit reticulocyte lysates. It was confirmed that RNA 4 codes for a polypeptide of approx. 19000 and RNA 3 is translated into a polypeptide of 32000 daltons. RNAs 2 and 1 were each shown to code for polypeptides of approx. 105000 daltons. Several smaller polypeptides were also synthesized and these are probably incomplete products. Of the two minor RNA components (RNAs 5 and 6) found in isolated virions only RNA 5 could be translated, the major product being approx. 68000 daltons.

Received 12 December 1978; accepted 20 March 1979.