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Sequence Studies of Poliovirus RNA. IV. Nucleotide Sequence Complexities of Poliovirus Type 1, Type 2 and Two Type 1 Defective Interfering Particles RNAs, and Fingerprint of the Poliovirus Type 3 Genome

Akio Nomoto

Department of Microbiology, School of Basic Health Sciences, State University of New York at Stony Brook, Stony Brook, L.I., New York 11794, U.S.A.

The ³²P-labelled genomes of poliovirus type 1, 2 and 3 have been digested with RNase T1 and the products separated by two-dimensional gel electrophoresis. All three fingerprints differ in the separation pattern of the large oligonucleotides. The molar yields of the large RNase T1-resistant oligonucleotides of type 1 and type 2 RNA of poliovirus RNA are close to one. By comparing the yields of these oligonucleotides to the amount of RNA from which they originated, the chain length of type 1 poliovirus RNA was found to be 7851±567 nucleotides (mol. wt. 2.66±0.19 x 10⁶) and that of poliovirus type 2, 8181±578 nucleotides (mol. wt. 2.77±0.19 x 10⁶). The chain length of two defective interfering particle (DI) RNAs of poliovirus type 1 were determined to be 7042±999 nucleotides for DI(1) and 6639±674 nucleotides for DI(2).

^* Present address: 3837 Simpson Stuart Road, Life Science Department, Bishop College, Dallas, Texas 75241, U.S.A.

Present address: Department of Public Health, School of Pharmaceutical Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, Japan.

To whom correspondence should be addressed.

Received 29 November 1978; accepted 12 January 1979.

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