HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bellini, W. J. Articles by McFarlin, D. E. Search for Related Content PubMed PubMed Citation Articles by Bellini, W. J. Articles by McFarlin, D. E. Agricola Articles by Bellini, W. J. Articles by McFarlin, D. E.

Purification of Measles Virus with Preservation of Infectivity and Antigenicity

Neuroimmunology Branch, National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Md, U.S.A.

Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.

Received 23 August 1978; accepted 4 December 1978.

This article has been cited by other articles:

				R. K. Plemper, K. J. Erlandson, A. S. Lakdawala, A. Sun, A. Prussia, J. Boonsombat, E. Aki-Sener, I. Yalcin, I. Yildiz, O. Temiz-Arpaci, et al. A target site for template-based design of measles virus entry inhibitors PNAS, April 13, 2004; 101(15): 5628 - 5633. [Abstract] [Full Text] [PDF]