HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Galloway, D. A. Articles by Sambrook, J. Search for Related Content PubMed PubMed Citation Articles by Galloway, D. A. Articles by Sambrook, J. Agricola Articles by Galloway, D. A. Articles by Sambrook, J.

Transformation of Rat Cells by the Hybrid Virus Ad2²⁺ HEY

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, U.S.A.

A set of four isogenic rat cell lines transformed by Ad2²⁺ HEY have been studied. While all of the cell lines synthesize SV40 T antigen, only one expresses adenovirus 2 T antigen: none expresses SV40 V antigen or adenovirus 2 fibre antigen. Three cell lines contain 1 to 2 virus equivalents of SV40 and adenoviral sequences per diploid quantity of rat cell DNA and the fourth line contains five copies of SV40 and 20 copies of the adenovirus genome. At least three of the cell lines contain DNA sequences from the helper adenovirus 2 in addition to sequences from the Ad²⁺ HEY genome. The patterns of integrated virus sequences are complex suggesting multiple insertions of both adenovirus and SV40 DNA sequences. SV40 can be rescued from three cell lines by fusion with permissive cells.

^* Present address: Fred Hutchinson Cancer Research Centre, Seattle, Wa., U.S.A.

Received 2 June 1978; accepted 24 August 1978.