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Lipid and Protein Organization in Vesicular Stomatitis and Sindbis Viruses

Animal Virus Research Institute, Pirbright, Surrey, England

M. C. Horzinek

Institute of Virology State University, Utrecht, Netherlands

Vesicular stomatitis virus was disrupted by 0.1% SDS into RNA, lipid and the individual virus polypeptides. If the virus was first treated with 0.4% formaldehyde, the particles retained their bullet shape on treatment with 0.1% SDS, although they were extensively penetrated by phosphotungstic acid. Radiochemical analysis using virus labelled with [¹⁴C]-amino acids or [¹⁴C]-choline or [³H]-glucosamine indicated that about 82% of the glycoprotein and about 96% of the protein was retained in the formaldehyde pre-treated particles, which were then treated with SDS, although more than 90% of the lipid was lost. With Sinbis virus also the overall structure of the particles was retained when the virus which had been pre-treated with formaldehyde was treated with 0.1% SDS. With this virus, about 60% of the glycoprotein was retained in the SDS-treated formalinized particles despite the loss of all the lipid. These results suggest that the surface projections are located in close proximity to the core protein.

Received 18 October 1973; accepted 27 November 1973.