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Capsid Polypeptides of Two Viruses Isolated from Penicillium stoloniferum

Department of Biochemistry Imperial College of Science and Technology, London SW7 2AY, U.K.

The capsid polypeptides of Penicillium stoloniferum virus S (PsV-S) and P. stoloniferum virus F (PsV-F) were examined by SDS-polyacrylamide gel electrophoresis. PsV-S contained one major (S2) and one minor (S1) polypeptide with mol. wt. 42500 and 55500, while PsV-F contained one major (F2) and one minor (F1) polypeptide with mol. wt. 47000 and 59000, respectively. Mol. wt. of S2 and F2 polypeptides calculated from amino acid analyses were 41500 and 48000, respectively. PsV-S capsid was estimated to contain 120 molecules of polypeptide S2 and 1 molecule of polypeptide S1.

Received 25 September 1973; accepted 15 November 1973.