HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Morser, M. J. Articles by Burke, D. C. Search for Related Content PubMed PubMed Citation Articles by Morser, M. J. Articles by Burke, D. C. Agricola Articles by Morser, M. J. Articles by Burke, D. C.

Cleavage of Virus-specified Polypeptides in Cells Infected with Semliki Forest Virus

Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England

Several new virus-specified polypeptides of high mol. wt. were detected in cells infected with Semliki Forest virus by use of pulse-chase methods in cells which had been either incubated at an elevated temperature in the presence of tosyl-L-phenylalanylchloromethane or treated with an inhibitor of glycoprotein synthesis, or an inhibitor of proteolytic enzyme activity, or five amino acid analogues, or an inhibitor of protein synthesis. The new polypeptides had estimated mol. wt. of 165000, 127000 and 105000 and were shown to be converted to lower mol. wt. polypeptides in pulse-chase experiments. These polypeptides, together with known non-structural polypeptides of mol. wt. of 97000, 78000 and 63000 are incorporated into a proposed cleavage mechanism for the synthesis of the virus structural proteins.

Received 16 August 1973; accepted 2 November 1973.